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- Product number
- NBP1-44073 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-44073, RRID:AB_2059263
- Product name
- Rabbit Polyclonal ARHGAP22 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. The NBP1-44073 antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22->A mutation but not the S397->A mutation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.07 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references p68RacGAP is a novel GTPase-activating protein that interacts with vascular endothelial zinc finger-1 and modulates endothelial cell capillary formation.
Aitsebaomo J, Wennerberg K, Der CJ, Zhang C, Kedar V, Moser M, Kingsley-Kallesen ML, Zeng GQ, Patterson C
The Journal of biological chemistry 2004 Apr 23;279(17):17963-72
The Journal of biological chemistry 2004 Apr 23;279(17):17963-72
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Western Blot: ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397->A mutation but not the S22->A mutation, while NBP1-44073 antibody recognizes the pS22->A mutation and not the pS397->A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.
