Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C745016 - Provider product page
- Provider
- LSBio
- Product name
- AGAP4 Antibody LS-C745016
- Antibody type
- Polyclonal
- Description
- Protein A affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit Anti-AGAP4 Antibody. Lane 1: HeLA cells treated with HA/AGAP4. Lane 2: HeLA cells control. Lane 3: HeLA cells treated with AGAP4/siRNA. Load: 10 µg per lane. Primary antibody: AGAP4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 71 kDa, 71 kDa for AGAP4. Other band(s): 42kDa Actin loading control.