PA5-77162
antibody from Invitrogen Antibodies
Targeting: TRMT9B
C8orf79, FLJ36980, hTRM9L, KIAA1456, TRM9L
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-77162 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIAA1456 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references CHIKV infection reprograms codon optimality to favor viral RNA translation by altering the tRNA epitranscriptome.
Jungfleisch J, Böttcher R, Talló-Parra M, Pérez-Vilaró G, Merits A, Novoa EM, Díez J
Nature communications 2022 Aug 11;13(1):4725
Nature communications 2022 Aug 11;13(1):4725
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KIAA1456 in extracts of various cell lines. Samples were incubated with KIAA1456 polyclonal antibody (Product # PA5-77162).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KIAA1456 in extracts of various cell lines. Samples were incubated with KIAA1456 polyclonal antibody (Product # PA5-77162).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of KIAA1456 in A549 cells. The samples were probed with a KIAA1456 polyclonal antibody (Product # PA5-77047) followed by DAPI to stain cell nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIAA1456 in paraffin-embedded rat lung. Samples were incubated with KIAA1456 polyclonal antibody (Product # PA5-77162).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KIAA1456 in paraffin-embedded rat lung. Samples were incubated with KIAA1456 polyclonal antibody (Product # PA5-77162).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Effect of KIAA1456 overexpression and depletion on mcm 5 U and mcm 5 s 2 U modification levels and on CHIKV infection. a Western blot showing KIAA1456 protein upregulation upon CHIKV infection. Values denote relative KIAA1456 expression normalized by tubulin expression calculated from 3 independent replicates and expressed as mean +- SEM. b Western blot showing KIAA1456 protein levels under plasmid-driven overexpression (KIAA1456-OE) and CHIKV infection conditions. Values represent relative KIAA1456 expression normalized by vinculin expression. A representative example from two independent replicates is shown. Note, that to be able to compare expression levels, 1:5 dilutions of the KIAA1456-OE overexpression samples were loaded. c Relative mcm 5 U and mcm 5 s 2 U modification levels in mock-infected cells, CHIKV-infected cells and in cells overexpressing KIAA1456 (KIAA1456-OE) determined by mass LC-MS-MS; bars represent the mean value +- SEM of 4 (in the case of mcm 5 after KIAA1456-OE) respectively 5 (in the remaining cases) independent replicates. p -values: mcm 5 - mock vs CHIKV: 0.011, mcm5 - mock vs KIAA-OE: 0.016. d Relative amount of CHIKV RNA levels ( p -value 0.0046) and ( e ) of CHIKV capsid protein levels in CHIKV-infected cells transfected with an empty plasmid or with a plasmid overexpressing KIAA1456 (KIAA1456-OE) determined by qPCR; bars represent the mean value +- SEM of 5 (empty) and 4 (KIAA-OE) independent replicates. CHIKV RNA and capsid protein expression
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Effect of DENV and HCV infection on KIAA1456 protein levels and mcm 5 and mcm 5 s 2 U levels. a Western blot showing KIAA1456 protein expression levels upon DENV and HCV infection. Values denote KIAA1456 expression normalized by tubulin expression relative to uninfected cells calculated from 3 independent replicates and expressed as mean +- SEM. b as in Fig. 5c but upon DENV and HCV infection. Bars represent the mean value +- SEM of 5 independent replicates ( p -value 0.007). Statistical analyses were performed using the two-tailed t -test. * p < 0.05.