Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F48401 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-Histone H3 Antibody (pS10)
- Antibody type
- Polyclonal
- Antigen
- This phospho-Histone H3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS10 of human HIST1H3A.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the phospho-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HeLa cell line, untreated or treated with Calyculin A (100ng/ml), using phospho-Histone H3 antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HeLa cell line, untreated or treated with FBS + Calyculin A (100ng/ml), using phospho-Histone H3 antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HeLa cell line using phospho-Histone H3 antibody at 1:1000.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells, untreated or treated with FBS + Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells, untreated or treated with FBS and Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of phospho-Histone H3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.