F54560

antibody from NSJ Bioreagents

Targeting: H2BC11 H2B/r, H2BFR, HIST1H2BJ

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54560

Provider

NSJ Bioreagents

Product name

Histone H2B type 1-J Antibody / HIST1H2BJ

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Zebrafish

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the HIST1H2BJ antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) ZR4, 2) whole zebrafish and 3) zebrafish muscle lysate with HIST1H2BJ antibody. Predicted molecular weight ~14 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) CEM, 2) HL60 and 3) NCI-H460 cell lysate with HIST1H2BJ antibody. Predicted molecular weight ~14 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human hepatocarcinoma tissue with HIST1H2BJ antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human CEM cells with HIST1H2BJ antibody; Blue=isotype control, Green= HIST1H2BJ antibody.