500-P241-100UG

antibody from Invitrogen Antibodies

Targeting: DEFB103A DEFB-3, DEFB103, DEFB3, HBD-3, HBD3, HBP-3, HBP3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P241-100UG

Provider

Invitrogen Antibodies

Product name

beta-3 Defensin Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK.

Concentration

0.1-1.0 mg/mL

References

Submitted references

Human beta-defensin 3 contributes to the carcinogenesis of cervical cancer via activation of NF-κB signaling.

Xu D, Zhang B, Liao C, Zhang W, Wang W, Chang Y, Shao Y
Oncotarget 2016 Nov 15;7(46):75902-75913

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Bitter and sweet taste receptors regulate human upper respiratory innate immunity.

Lee RJ, Kofonow JM, Rosen PL, Siebert AP, Chen B, Doghramji L, Xiong G, Adappa ND, Palmer JN, Kennedy DW, Kreindler JL, Margolskee RF, Cohen NA
The Journal of clinical investigation 2014 Mar;124(3):1393-405

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IL-1F5, -F6, -F8, and -F9: a novel IL-1 family signaling system that is active in psoriasis and promotes keratinocyte antimicrobial peptide expression.

Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2613-22

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An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2, a model for tumorigenesis.

Jin G, Kawsar HI, Hirsch SA, Zeng C, Jia X, Feng Z, Ghosh SK, Zheng QY, Zhou A, McIntyre TM, Weinberg A
PloS one 2010 Jun 8;5(6):e10993

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UV-B radiation induces the expression of antimicrobial peptides in human keratinocytes in vitro and in vivo.

Gläser R, Navid F, Schuller W, Jantschitsch C, Harder J, Schröder JM, Schwarz A, Schwarz T
The Journal of allergy and clinical immunology 2009 May;123(5):1117-23

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Association of a genetic polymorphism (-44 C/G SNP) in the human DEFB1 gene with expression and inducibility of multiple beta-defensins in gingival keratinocytes.

Kalus AA, Fredericks LP, Hacker BM, Dommisch H, Presland RB, Kimball JR, Dale BA
BMC oral health 2009 Aug 27;9:21

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Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity.

Ghosh SK, Gerken TA, Schneider KM, Feng Z, McCormick TS, Weinberg A
Clinical chemistry 2007 Apr;53(4):757-65

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human BD-3 by Western Blot analysis beta-3 Defensin Polyclonal Antibody (Product # 500-P241-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human BD-3 by Western Blot analysis beta-3 Defensin Polyclonal Antibody (Product # 500-P241-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human BD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of beta-3 Defensin Polyclonal Antibody (Product # 500-P241-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech beta-3 Defensin Polyclonal Antibody, Biotin (Product # 500-P241BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-3.