Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-29986 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Centrin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, 293T, A431.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Centrin-1 using Various whole cell extracts (30 µg). Samples were loaded onto a 15% SDS-PAGE gel and probed with a Centrin-1 polyclonal antibody (Product # PA5-29986) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Centrin 1 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Centrin 1 Polyclonal Antibody (Product # PA5-29986) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Centrin 1 Polyclonal Antibody (Product # PA5-29986). Sample (30 µg of whole cell lysate). Lane A: JurKat. 12% SDS PAGE. Centrin-1 antibody . Centrin 1 Polyclonal Antibody (Product # PA5-29986) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Centrin 1 was performed in A431 cells fixed in ice cold MeOH for 5 min. Green: Centrin 1 Polyclonal Antibody (Product # PA5 29986) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 µg of Centrin-1 antibody [N1C3] (Product # PA5-29986). Western blot analysis was performed using Centrin-1 antibody [N1C3] (Product # PA5-29986) diluted at 1:500.