Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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Validation data
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- Product number
- GTX11257 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX11257, RRID:AB_368851
- Product name
- Centrin 1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Lysate of HEK-293T cells overexpressing human centrin-1 was separated on SDS-PAGE, blotted with Anti-Centrin (GTX11257) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 0.3 μg/mL 2. Antibody 0.3 μg/mL + centrin1 immunizing peptide (human, 157-172)
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- GeneTex (provider)
- Main image
- Experimental details
- Lysates of HT-29 (lanes 1,2), HEK-293T (lanes (3-4) and HeLa (lanes 5-6) cells were separated on SDS-PAGE, blotted with 0.3 μg/mL Anti-Centrin (GTX11257) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1,3,5. Antibody 0.3 μg/mL 2,4,6. Antibody 0.3 μg/mL + centrin1 immunizing peptide (human, 157-172)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NIH3T3 cells were fixed and stained with 6 μg/mL Anti-Centrin (GTX11257). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjμgate. Nuclear staining with DAPI.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa cells were fixed and stained with 6 μg/mL Anti-Centrin (GTX11257). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjμgate. Nuclear staining with DAPI.