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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- TA501452 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501452, RRID:AB_11124038
- Product name
- TMEM80 mouse monoclonal antibody, clone OTI2B7 (formerly 2B7)
- Antibody type
- Monoclonal
- Description
- TMEM80 mouse monoclonal antibody, clone OTI2B7 (formerly 2B7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TMEM80
- Isotype
- IgG
- Antibody clone number
- OTI2B7
- Vial size
- 100 µl
- Concentration
- 0.81 mg/ml
Submitted references MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition Zone.
Li C, Jensen VL, Park K, Kennedy J, Garcia-Gonzalo FR, Romani M, De Mori R, Bruel AL, Gaillard D, Doray B, Lopez E, Rivière JB, Faivre L, Thauvin-Robinet C, Reiter JF, Blacque OE, Valente EM, Leroux MR
PLoS biology 2016 Mar;14(3):e1002416
PLoS biology 2016 Mar;14(3):e1002416
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM80 (RC202288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM80.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TMEM80 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TMEM80 mouse monoclonal antibody (TA501452) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TMEM80(RC202288).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC202288 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TMEM80 antibody(TA501452), and then analyzed by flow cytometry.
- Validation comment
- FC
