Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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Validation data
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- Product number
- 22249-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBM15B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This RBM15B antibody was produced by immunizing animals with the C-terminus of RBM15B, which is homologous to a short form of RBM15B (563 aa, ~70 kDa), thus this antibody can recognize full length of RBM15B (~100 kDa) and splice-one (70 kDa).
- Concentration
- 0.27 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RBM15B (IP:22249-1-AP, 4ug; Detection:22249-1-AP 1:500) with HeLa cells lysate 2500ug.