Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00003306-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003306-M02, RRID:AB_489707
- Product name
- HSPA2 monoclonal antibody (M02), clone 3H7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant HSPA2.
- Antigen sequence
MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQG
NRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFD
AKRLIGRKFGDATVQSDMKHWPFRVVSEGGKPKVQ
VEYKGETKTFFPEEISSMVLTKMKEIAEAYLGGKV
HSAVITVPAYFNDSQRQATKDAGTITGLNVLRIIN
EPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDV
SILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLA
EEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSS
TQASIEIDSLYEGVDFYTSITRARFEELNADLFRG
TLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQ
KLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIG
DKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRN
TTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKD
NNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILN
VTAADKSTGKENKITITNDKGRLSKDDIDRMVQEA
ERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEK
LRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEY
EHKQKELERVCNPIISKLYQGGPGGGSGGGGSGAS
GGPTIEEVD- Isotype
- IgG
- Antibody clone number
- 3H7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSPA2 monoclonal antibody (M02), clone 3H7 Western Blot analysis of HSPA2 expression in HepG2 ( Cat # L019V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HSPA2 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to HSPA2 on HepG2 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HSPA2 transfected lysate using anti-HSPA2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HSPA2 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
