- HPA000798 - Atlas Antibodies HSPA2 antibody

Submitted references Expression, function, and regulation of the testis-enriched heat shock HSPA2 gene in rodents and humans.

Scieglinska D, Krawczyk Z
Cell stress & chaperones 2015 Mar;20(2):221-35

HSP70-binding protein HSPBP1 regulates chaperone expression at a posttranslational level and is essential for spermatogenesis.

Rogon C, Ulbricht A, Hesse M, Alberti S, Vijayaraj P, Best D, Adams IR, Magin TM, Fleischmann BK, Höhfeld J
Molecular biology of the cell 2014 Aug 1;25(15):2260-71

The molecular chaperone Hsp90α is required for meiotic progression of spermatocytes beyond pachytene in the mouse.

Grad I, Cederroth CR, Walicki J, Grey C, Barluenga S, Winssinger N, De Massy B, Nef S, Picard D
PloS one 2010 Dec 31;5(12):e15770

Systematically generated antibodies against human gene products: High throughput screening on sections from the rat nervous system

Mulder J, Wernérus H, Shi T, Pontén F, Hober S, Uhlén M, Hökfelt T
Neuroscience 2007 June;146(4):1689-1703

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
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Experimental details: Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSPA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human testis and pancreas tissues using HPA000798 antibody. Corresponding HSPA2 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in decidual cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining of mouse thalamus shows strong immunoreactivity in neurons.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining of mouse somatosensory cortex shows strong immunoreactivity in neuronal cell bodies.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining of mouse amygdala shows positivity in neurons.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows moderate immunoreactivity in neuropil and neuronal cell bodies.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebellum shows strong immunoreactivity in a subset of glial cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in glial cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of mouse brain shows moderate to strong positivity in more than 75 % of neurons in the thalamus.
Sample type: MOUSE

HPA000798

Antibody data