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antibody from Invitrogen Antibodies
Targeting: MSR1
CD204, SCARA1, SR-A, SR-AI, SR-AII, SR-AIII
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-22957 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD204 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat, canine, non-human primate, bovine, and equine based on homology. Suggested positive control: antigen standard for MSR1 (transient overexpression lysate), human liver protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis.
Hamasaki S, Kobori T, Yamazaki Y, Kitaura A, Niwa A, Nishinaka T, Nishibori M, Mori S, Nakao S, Takahashi H
Scientific reports 2018 Apr 12;8(1):5901
Scientific reports 2018 Apr 12;8(1):5901
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD204 using a polyclonal antibody (Product # PA5-22957).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD204 in human liver. Sample was incubated in CD204 polyclonal antibody (Product # PA5-22957).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD204 using a polyclonal antibody (Product # PA5-22957).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 AGE-2 and AGE-3 effects on scavenger receptor expression. ( a ) RAW264.7 cells seeded at 2 x 10 5 per well incubated for 1 h with 200 mug/ml AGE-2, AGE-3, or BSA were stained with specific FITC-labelled Abs against CD163 or CD206, specific PE-labelled CD204 or LOX-1, and APC-labelled CD36 or RAGE, and relative mean fluorescence intensities (MFIs) determined by flow cytometry (n = 6). Each column represents respective surface protein MFIs relative to medium only (Untreated, Unt) after 1 h incubation, arbitrarily defined as 100%. Data are presented as the means +- SEM and analysed using one-way ANOVA followed by Tukey's test. *** p < 0.001, ** p < 0.01 compared with the Unt value. ( b ) For RAW264.7 cells seeded at 2 x 10 5 per well incubated with increasing concentrations AGE-2, AGE-3, and BSA (0.2-200 mug/ml) for 1 or 4 h, relative CD204 MFIs were determined by flow cytometry (n = 4-6). Each column represents MFIs relative to Unt after 1 or 4 h incubation, arbitrarily defined as 100%. Data are presented as the means +- SEM and analysed using one-way ANOVA followed by Tukey's test. *** p < 0.001, * p < 0.05 compared with Unt. Insert in 1 h shows overlay of representative monoparametric histogram of CD204 expression in RAW264.7 cells incubated with medium only (Untreated; greyscale), 200 mug/ml BSA (orange), AGE-2 (blue), or AGE-3 (red line). ( c ) RAW264.7 cells seeded at 4 x 10 5 per well were cultured for 4 h with 200 mug/ml Alexa Fluor 488 (green)-labelled BSA, AGE
