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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF2708 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SR-AI/MSR Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SR-AI/MSR in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SR-AI is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P21757- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pattern recognition scavenger receptor CD204 attenuates Toll-like receptor 4-induced NF-kappaB activation by directly inhibiting ubiquitination of tumor necrosis factor (TNF) receptor-associated factor 6.
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Yu X, Yi H, Guo C, Zuo D, Wang Y, Kim HL, Subjeck JR, Wang XY
The Journal of biological chemistry 2011 May 27;286(21):18795-806
The Journal of biological chemistry 2011 May 27;286(21):18795-806
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
Circulation research 2009 Aug 28;105(5):500-10
Circulation research 2009 Aug 28;105(5):500-10
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SR-AI/MSR by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human SR-AI/MSR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2708) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SR-AI/MSR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SR-AI/MSR in THP-1 Human Cell Line. SR-AI/MSR was detected in immersion fixed THP-1 human acute monocytic leukemia cell line untreated (right panel) and treated with PMA (left panel) using Goat Anti-Human SR-AI/MSR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2708) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
