PAB21526

antibody from Abnova Corporation

Targeting: MRPL12 MRPL7, MRPL7/L12, RPML12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21526

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21526, RRID:AB_10967391

Product name

MRPL12 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MRPL12.

Antigen sequence

SLTRFAIYETVRDRVAKGSQGPLPFHEKVLLGSVS
GLAGGFVGTPADLVNVRMQNDVKLPQGQRRNYAHA
L


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with MRPL12 polyclonal antibody (Cat # PAB21526).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with MRPL12 polyclonal antibody (Cat # PAB21526) at 1-4 ug/mL dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with MRPL12 polyclonal antibody (Cat # PAB21526) shows strong cytopmasmic positivity with granular pattern in cells in tubules and cells in glomeruli at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)