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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10690 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10690, RRID:AB_11190481
- Product name
- HSPB1 monoclonal antibody, clone 5D7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant HSPB1.
- Isotype
- IgG
- Antibody clone number
- 5D7
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) against HeLa (1) , A-549 (2) , Jurkat (3) , A-431 (4) , HEK293 (5) , HepG2 (6) and PC-12 (7) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) .
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissues (A) , esophageal cancer tissues (B) , breast cancer tissues (C) and cardiac muscle tissues (D) using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using HSPB1 monoclonal antibody, clone 5D7 (Cat # MAB10690) (green) and negative control (purple) .
- Validation comment
- Flow Cytometry
