LS-C330863

antibody from LSBio

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C330863

Provider

LSBio

Product name

HSPB1 / HSP27 Antibody LS-C330863

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using HSPB1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug HSPB1 antibody. Western blot was performed from the immunoprecipitate using HSPB1 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug HSPB1 antibody. Western blot was performed from the immunoprecipitate using HSPB1 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug HSPB1 antibody. Western blot was performed from the immunoprecipitate using HSPB1 antibodyat a dilition of 1:1000.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibodyat dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibodyat dilution of 1:100 (40x lens).