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Western blot
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- GTX101145 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101145, RRID:AB_1240967
- Product name
- HSP27 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
5-Methoxytryptophan-dependent protection of cardiomyocytes from heart ischemia reperfusion injury.
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Proteomic analysis of proteins responsible for the development of doxorubicin resistance in human uterine cancer cells.
Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58
Oncotarget 2016 Feb 2;7(5):6146-58
5-Methoxytryptophan-dependent protection of cardiomyocytes from heart ischemia reperfusion injury.
Chou HC, Chan HL
Archives of biochemistry and biophysics 2014 Feb 1;543:15-22
Archives of biochemistry and biophysics 2014 Feb 1;543:15-22
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Huang HJ, Lin CC, Chou HC, Chen YW, Lin ST, Lin YC, Lin DY, Lyu KW, Chan HL
Molecular bioSystems 2014 Dec;10(12):3086-100
Molecular bioSystems 2014 Dec;10(12):3086-100
Proteomic analysis of proteins responsible for the development of doxorubicin resistance in human uterine cancer cells.
Lin ST, Chou HC, Chang SJ, Chen YW, Lyu PC, Wang WC, Chang MD, Chan HL
Journal of proteomics 2012 Oct 22;75(18):5822-47
Journal of proteomics 2012 Oct 22;75(18):5822-47
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HT-29 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 C: Hela D: Hep G2 (GTX27900) 12% SDS PAGE GTX101145 diluted at 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse liver 12% SDS PAGE GTX101145 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP27 antibody detects HSP27 protein by western blot analysis.A. 30 ?g A431 whole cell extractB. 30 ?g HeLa whole cell extract12 % SDS-PAGEHSP27 antibody (GTX101145) dilution: 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and HSP27 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HT-29 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and HSP27 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HSP27 protein stained by HSP27 antibody (GTX101145) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ?g of HSP27 (GTX101145) 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody (GTX101145) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 £gg of HSP27 antibody (GTX101145).Western blot analysis was performed using HSP27 antibody (GTX101145).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSP27 antibody detects HSP27 protein at cytoplasm in human lung papillary carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung papillary carcinoma. HSP27 antibody (GTX101145) diluted at 1:500.
