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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- bs-0730R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-0730R, RRID:AB_10857781
- Product name
- HSP27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic peptide derived from human HSP27
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression.
Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprung's disease.
Liu Z, Duan ZJ, Chang JY, Zhang ZF, Chu R, Li YL, Dai KH, Mo GQ, Chang QY
PloS one 2014;9(6):e98560
PloS one 2014;9(6):e98560
Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprung's disease.
Gao H, Liu X, Chen D, Lv L, Wu M, Mi J, Wang W
International journal of experimental pathology 2014 Jun;95(3):229-37
International journal of experimental pathology 2014 Jun;95(3):229-37
No comments: Submit comment
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 27kD. Observed band size:27kD.
- Sample type
- Mouse
- Other comments
- Liver
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
- Sample type
- Rat
- Other comments
- Brain
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated(bs-0730R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
- Sample type
- Rat
- Other comments
- Brain
