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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Protein array [1]
- Other assay [1]
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- Product number
- V7422 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HSP27 Antibody / HSPB1
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- CPTC-HSPB1-2
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate tissue with HSP27 antibody (clone CPTC-HSPB1-2). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HSP27 antibody (clone CPTC-HSPB1-2). These results demonstrate the foremost specificity of the CPTC-HSPB1-2 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone CPTC-HSPB1-2) as confirmation of integrity and purity.
