LS-B16406

antibody from LSBio

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Enhanced validation)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B16406

Provider

LSBio

Product name

IHC-plus™ HSPB1 / HSP27 Antibody LS-B16406

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE HSPB1 antibody diluted at 1:1000

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE HSPB1 antibody diluted at 1:1000

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ug of HSP27 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ug of HSP27 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ug of HSP27 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ug of HSP27 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.