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Learn200-301-243
antibody from Rockland Immunochemicals, Inc.
Targeting: HSPB1
CMT2F, Hs.76067, Hsp25, HSP27, HSP28
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 200-301-243 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-243, RRID:AB_2120629
- Product name
- Anti-HSP27 (MOUSE) Monoclonal Antibody - 200-301-243
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000. A single band corresponding to human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human HSP27. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot
