Explore
Validate
LearnADI-SPA-800-D
antibody from Enzo Life Sciences
Targeting: HSPB1
CMT2F, Hs.76067, Hsp25, HSP27, HSP28
Western blot
ELISA
Immunocytochemistry Immunoprecipitation Immunohistochemistry Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ADI-SPA-800-D - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-SPA-800-D, RRID:AB_2039215
- Product name
- HSP27 monoclonal antibody (G3.1)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Protein G affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- G3.1
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Glutathione Levels and Susceptibility to Chemically Induced Injury in Two Human Prostate Cancer Cell Lines.
HSP27 is involved in the pathogenesis of kidney tubulointerstitial fibrosis.
Chemical and biological approaches synergize to ameliorate protein-folding diseases.
Cytotoxicity effects induced by Zearalenone metabolites, alpha Zearalenol and beta Zearalenol, on cultured Vero cells.
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans.
Modulation of heat-shock protein 27 (Hsp27) anti-apoptotic activity by methylglyoxal modification.
Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability.
Subcorneal colocalization of the small heat shock protein, hsp27, with keratins and proteins of the cornified cell envelope.
Estrogenic activity of procymidone in primary cultured rainbow trout hepatocytes (Oncorhynchus mykiss).
Effect of the serine protease inhibitor N-tosyl-l-phenylalanine-chloromethyl ketone (TPCK) on MCF-7 mammary tumour cells growth and differentiation.
Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. Relationship to myogenic regulatory factors.
Lash LH, Putt DA, Jankovich AD
Molecules (Basel, Switzerland) 2015 Jun 5;20(6):10399-414
Molecules (Basel, Switzerland) 2015 Jun 5;20(6):10399-414
HSP27 is involved in the pathogenesis of kidney tubulointerstitial fibrosis.
Vidyasagar A, Reese S, Acun Z, Hullett D, Djamali A
American journal of physiology. Renal physiology 2008 Sep;295(3):F707-16
American journal of physiology. Renal physiology 2008 Sep;295(3):F707-16
Chemical and biological approaches synergize to ameliorate protein-folding diseases.
Mu TW, Ong DS, Wang YJ, Balch WE, Yates JR 3rd, Segatori L, Kelly JW
Cell 2008 Sep 5;134(5):769-81
Cell 2008 Sep 5;134(5):769-81
Cytotoxicity effects induced by Zearalenone metabolites, alpha Zearalenol and beta Zearalenol, on cultured Vero cells.
Othmen ZO, Golli EE, Abid-Essefi S, Bacha H
Toxicology 2008 Oct 30;252(1-3):72-7
Toxicology 2008 Oct 30;252(1-3):72-7
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans.
Paulsen G, Vissing K, Kalhovde JM, Ugelstad I, Bayer ML, Kadi F, Schjerling P, Hallén J, Raastad T
American journal of physiology. Regulatory, integrative and comparative physiology 2007 Aug;293(2):R844-53
American journal of physiology. Regulatory, integrative and comparative physiology 2007 Aug;293(2):R844-53
Modulation of heat-shock protein 27 (Hsp27) anti-apoptotic activity by methylglyoxal modification.
Sakamoto H, Mashima T, Yamamoto K, Tsuruo T
The Journal of biological chemistry 2002 Nov 29;277(48):45770-5
The Journal of biological chemistry 2002 Nov 29;277(48):45770-5
Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability.
Garcia JG, Wang P, Schaphorst KL, Becker PM, Borbiev T, Liu F, Birukova A, Jacobs K, Bogatcheva N, Verin AD
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jul;16(9):1064-76
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jul;16(9):1064-76
Subcorneal colocalization of the small heat shock protein, hsp27, with keratins and proteins of the cornified cell envelope.
Jonak C, Klosner G, Kokesch C, FOdinger D, HOnigsmann H, Trautinger F
The British journal of dermatology 2002 Jul;147(1):13-9
The British journal of dermatology 2002 Jul;147(1):13-9
Estrogenic activity of procymidone in primary cultured rainbow trout hepatocytes (Oncorhynchus mykiss).
Radice S, Ferraris M, Marabini L, Chiesara E
Toxicology in vitro : an international journal published in association with BIBRA 2002 Aug;16(4):475-80
Toxicology in vitro : an international journal published in association with BIBRA 2002 Aug;16(4):475-80
Effect of the serine protease inhibitor N-tosyl-l-phenylalanine-chloromethyl ketone (TPCK) on MCF-7 mammary tumour cells growth and differentiation.
Horman S, Del Bino G, Fokan D, Mosselmans R, Galand P
Cell biology international 2000;24(3):153-61
Cell biology international 2000;24(3):153-61
Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. Relationship to myogenic regulatory factors.
Neufer PD, Benjamin IJ
The Journal of biological chemistry 1996 Sep 27;271(39):24089-95
The Journal of biological chemistry 1996 Sep 27;271(39):24089-95
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of HSP27, mAb (G3.1) (Prod. No. ADI-SPA-800). Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: Vero (heat shocked), Lane 4: HSP27 (human) (recombinant) (Prod. No. ADI-SPP-715).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human skeletal muscle tissue stained with HSP27, mAb (G3.1) at 10µg/ml.
