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LearnNB100-338
antibody from Novus Biologicals
Targeting: NDC80
HEC, HEC1, hsNDC80, KNTC2, TID3
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [44]
- Comments [0]
- Validations
- Western blot [3]
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- Product number
- NB100-338 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-338, RRID:AB_10000917
- Product name
- Mouse Monoclonal NDC80 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The Hec1/Ndc80 tail domain is required for force generation at kinetochores, but is dispensable for kinetochore-microtubule attachment formation and Ska complex recruitment.
PLK1 has tumor-suppressive potential in APC-truncated colon cancer cells.
Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics.
SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability.
Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments.
The spindle and kinetochore-associated (Ska) complex enhances binding of the anaphase-promoting complex/cyclosome (APC/C) to chromosomes and promotes mitotic exit.
A small compound targeting TACC3 revealed its different spatiotemporal contributions for spindle assembly in cancer cells.
Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation.
The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.
A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction.
A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
P190B RhoGAP Regulates Chromosome Segregation in Cancer Cells.
Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle.
Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores.
Chromosome missegregation in human cells arises through specific types of kinetochore-microtubule attachment errors.
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore.
The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis.
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement.
The MIS12 complex is a protein interaction hub for outer kinetochore assembly.
A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds.
Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication.
Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments.
Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1.
Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules.
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal.
Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1).
Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain.
MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.
FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.
DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement.
PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.
Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture.
Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore.
The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK.
Wimbish RT, DeLuca KF, Mick JE, Himes J, Jiménez-Sánchez I, Jeyaprakash AA, DeLuca JG
Molecular biology of the cell 2020 Jul 1;31(14):1453-1473
Molecular biology of the cell 2020 Jul 1;31(14):1453-1473
PLK1 has tumor-suppressive potential in APC-truncated colon cancer cells.
Raab M, Sanhaji M, Matthess Y, Hörlin A, Lorenz I, Dötsch C, Habbe N, Waidmann O, Kurunci-Csacsko E, Firestein R, Becker S, Strebhardt K
Nature communications 2018 Mar 16;9(1):1106
Nature communications 2018 Mar 16;9(1):1106
Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics.
DeLuca KF, Meppelink A, Broad AJ, Mick JE, Peersen OB, Pektas S, Lens SMA, DeLuca JG
The Journal of cell biology 2018 Jan 2;217(1):163-177
The Journal of cell biology 2018 Jan 2;217(1):163-177
SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
Niikura Y, Kitagawa R, Ogi H, Kitagawa K
Cell cycle (Georgetown, Tex.) 2017 Sep 17;16(18):1683-1694
Cell cycle (Georgetown, Tex.) 2017 Sep 17;16(18):1683-1694
Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability.
Dumitru AMG, Rusin SF, Clark AEM, Kettenbach AN, Compton DA
eLife 2017 Nov 20;6
eLife 2017 Nov 20;6
Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
Tauchman EC, Boehm FJ, DeLuca JG
Nature communications 2015 Dec 1;6:10036
Nature communications 2015 Dec 1;6:10036
STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments.
Kleyman M, Kabeche L, Compton DA
Journal of cell science 2014 Oct 1;127(Pt 19):4225-33
Journal of cell science 2014 Oct 1;127(Pt 19):4225-33
The spindle and kinetochore-associated (Ska) complex enhances binding of the anaphase-promoting complex/cyclosome (APC/C) to chromosomes and promotes mitotic exit.
Sivakumar S, Daum JR, Tipton AR, Rankin S, Gorbsky GJ
Molecular biology of the cell 2014 Mar;25(5):594-605
Molecular biology of the cell 2014 Mar;25(5):594-605
A small compound targeting TACC3 revealed its different spatiotemporal contributions for spindle assembly in cancer cells.
Yao R, Kondoh Y, Natsume Y, Yamanaka H, Inoue M, Toki H, Takagi R, Shimizu T, Yamori T, Osada H, Noda T
Oncogene 2014 Aug 14;33(33):4242-52
Oncogene 2014 Aug 14;33(33):4242-52
Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation.
Kabeche L, Compton DA
Nature 2013 Oct 3;502(7469):110-3
Nature 2013 Oct 3;502(7469):110-3
The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.
Maier B, Kirsch M, Anderhub S, Zentgraf H, Krämer A
Cell cycle (Georgetown, Tex.) 2013 May 1;12(9):1457-71
Cell cycle (Georgetown, Tex.) 2013 May 1;12(9):1457-71
A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
De Antoni A, Maffini S, Knapp S, Musacchio A, Santaguida S
The Journal of cell biology 2012 Oct 15;199(2):269-84
The Journal of cell biology 2012 Oct 15;199(2):269-84
Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction.
Chan YW, Jeyaprakash AA, Nigg EA, Santamaria A
The Journal of cell biology 2012 Mar 5;196(5):563-71
The Journal of cell biology 2012 Mar 5;196(5):563-71
A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
Matson DR, Demirel PB, Stukenberg PT, Burke DJ
Genes & development 2012 Mar 15;26(6):542-7
Genes & development 2012 Mar 15;26(6):542-7
P190B RhoGAP Regulates Chromosome Segregation in Cancer Cells.
Hwang M, Peddibhotla S, McHenry P, Chang P, Yochum Z, Park KU, Sears JC, Vargo-Gogola T
Cancers 2012 Jun;4(2):475-89
Cancers 2012 Jun;4(2):475-89
Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
Krenn V, Wehenkel A, Li X, Santaguida S, Musacchio A
The Journal of cell biology 2012 Feb 20;196(4):451-67
The Journal of cell biology 2012 Feb 20;196(4):451-67
TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle.
Ma N, Titus J, Gable A, Ross JL, Wadsworth P
The Journal of cell biology 2011 Oct 3;195(1):87-98
The Journal of cell biology 2011 Oct 3;195(1):87-98
Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores.
Serio G, Margaria V, Jensen S, Oldani A, Bartek J, Bussolino F, Lanzetti L
Proceedings of the National Academy of Sciences of the United States of America 2011 Oct 18;108(42):17337-42
Proceedings of the National Academy of Sciences of the United States of America 2011 Oct 18;108(42):17337-42
Chromosome missegregation in human cells arises through specific types of kinetochore-microtubule attachment errors.
Thompson SL, Compton DA
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 1;108(44):17974-8
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 1;108(44):17974-8
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore.
Screpanti E, De Antoni A, Alushin GM, Petrovic A, Melis T, Nogales E, Musacchio A
Current biology : CB 2011 Mar 8;21(5):391-8
Current biology : CB 2011 Mar 8;21(5):391-8
The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis.
Sundin LJ, Guimaraes GJ, Deluca JG
Molecular biology of the cell 2011 Mar 15;22(6):759-68
Molecular biology of the cell 2011 Mar 15;22(6):759-68
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
Barnhart MC, Kuich PH, Stellfox ME, Ward JA, Bassett EA, Black BE, Foltz DR
The Journal of cell biology 2011 Jul 25;194(2):229-43
The Journal of cell biology 2011 Jul 25;194(2):229-43
Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
Santaguida S, Vernieri C, Villa F, Ciliberto A, Musacchio A
The EMBO journal 2011 Apr 20;30(8):1508-19
The EMBO journal 2011 Apr 20;30(8):1508-19
The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement.
Tooley JG, Miller SA, Stukenberg PT
Molecular biology of the cell 2011 Apr 15;22(8):1217-26
Molecular biology of the cell 2011 Apr 15;22(8):1217-26
The MIS12 complex is a protein interaction hub for outer kinetochore assembly.
Petrovic A, Pasqualato S, Dube P, Krenn V, Santaguida S, Cittaro D, Monzani S, Massimiliano L, Keller J, Tarricone A, Maiolica A, Stark H, Musacchio A
The Journal of cell biology 2010 Sep 6;190(5):835-52
The Journal of cell biology 2010 Sep 6;190(5):835-52
A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds.
Screpanti E, Santaguida S, Nguyen T, Silvestri R, Gussio R, Musacchio A, Hamel E, De Wulf P
PloS one 2010 Jul 15;5(7):e11603
PloS one 2010 Jul 15;5(7):e11603
Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.
Santaguida S, Tighe A, D'Alise AM, Taylor SS, Musacchio A
The Journal of cell biology 2010 Jul 12;190(1):73-87
The Journal of cell biology 2010 Jul 12;190(1):73-87
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Zeng K, Bastos RN, Barr FA, Gruneberg U
The Journal of cell biology 2010 Dec 27;191(7):1315-32
The Journal of cell biology 2010 Dec 27;191(7):1315-32
A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication.
Zanet J, Freije A, Ruiz M, Coulon V, Sanz JR, Chiesa J, Gandarillas A
PloS one 2010 Dec 20;5(12):e15701
PloS one 2010 Dec 20;5(12):e15701
Cells satisfy the mitotic checkpoint in Taxol, and do so faster in concentrations that stabilize syntelic attachments.
Yang Z, Kenny AE, Brito DA, Rieder CL
The Journal of cell biology 2009 Sep 7;186(5):675-84
The Journal of cell biology 2009 Sep 7;186(5):675-84
Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1.
Wu G, Wei R, Cheng E, Ngo B, Lee WH
Molecular biology of the cell 2009 Nov;20(22):4686-95
Molecular biology of the cell 2009 Nov;20(22):4686-95
Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules.
Cai S, Weaver LN, Ems-McClung SC, Walczak CE
Molecular biology of the cell 2009 Mar;20(5):1348-59
Molecular biology of the cell 2009 Mar;20(5):1348-59
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A
The Journal of cell biology 2009 Jun 1;185(5):859-74
The Journal of cell biology 2009 Jun 1;185(5):859-74
Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal.
Wu G, Qiu XL, Zhou L, Zhu J, Chamberlin R, Lau J, Chen PL, Lee WH
Cancer research 2008 Oct 15;68(20):8393-9
Cancer research 2008 Oct 15;68(20):8393-9
Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1).
Miller SA, Johnson ML, Stukenberg PT
Current biology : CB 2008 Nov 25;18(22):1785-91
Current biology : CB 2008 Nov 25;18(22):1785-91
Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain.
Wong J, Lerrigo R, Jang CY, Fang G
Molecular biology of the cell 2008 May;19(5):2083-91
Molecular biology of the cell 2008 May;19(5):2083-91
MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.
Barr AR, Gergely F
Molecular and cellular biology 2008 Dec;28(23):7199-211
Molecular and cellular biology 2008 Dec;28(23):7199-211
FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.
Zhu H, Coppinger JA, Jang CY, Yates JR 3rd, Fang G
The Journal of cell biology 2008 Dec 1;183(5):835-48
The Journal of cell biology 2008 Dec 1;183(5):835-48
DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement.
Jang CY, Wong J, Coppinger JA, Seki A, Yates JR 3rd, Fang G
The Journal of cell biology 2008 Apr 21;181(2):255-67
The Journal of cell biology 2008 Apr 21;181(2):255-67
PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.
Montembault E, Dutertre S, Prigent C, Giet R
The Journal of cell biology 2007 Nov 19;179(4):601-9
The Journal of cell biology 2007 Nov 19;179(4):601-9
Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
Klein UR, Nigg EA, Gruneberg U
Molecular biology of the cell 2006 Jun;17(6):2547-58
Molecular biology of the cell 2006 Jun;17(6):2547-58
HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture.
Wong J, Fang G
The Journal of cell biology 2006 Jun 19;173(6):879-91
The Journal of cell biology 2006 Jun 19;173(6):879-91
Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore.
Ciferri C, De Luca J, Monzani S, Ferrari KJ, Ristic D, Wyman C, Stark H, Kilmartin J, Salmon ED, Musacchio A
The Journal of biological chemistry 2005 Aug 12;280(32):29088-95
The Journal of biological chemistry 2005 Aug 12;280(32):29088-95
The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK.
Ganem NJ, Compton DA
The Journal of cell biology 2004 Aug 16;166(4):473-8
The Journal of cell biology 2004 Aug 16;166(4):473-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
