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Learn10281-1-AP
antibody from Invitrogen Antibodies
Targeting: LGALS3BP
90K, BTBD17B, CyCAP, gp90, M2BP, MAC-2-BP, TANGO10B
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- 10281-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LGALS3BP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LFWVWLLVA GTQGVNDGDM RLADGGATNQ GRVEIFYRGQ WGTVCDNLWD LTDASVVCRA LGFENATQAL GRAAFGQGSG PIMLDEVQCT GTEASLADCK SLGWLKSNCR HERDAGVVCT NETRSTHTLD LSRELSEALG QIFDSQRGCD LSISVNVQGE DALGFCGHTV ILTANLEAQA LWKEPGSNVT MSVDAECVPM VRDLLRYFYS RRIDITL (6-221 aa encoded by BC002403)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10281-1-AP (LGALS3BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP ( LGALS3BP Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP ( LGALS3BP Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:300) with HepG2 cells lysate 3480ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LGALS3BP (IP:10281-1-AP, 4 ug; Detection:10281-1-AP 1:400) with HEK-293 cells lysate 1000 ug.
