Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- A305-597A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM33 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TMEM33 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMEM33 antibody (Product # A305-597A-T; lot A305-597A-T-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TMEM33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TMEM33 antibody A305-597A-M (lot A305-597A-M-1) used for IP at 20 µl per reaction. TMEM33 was also immunoprecipitated by rabbit anti-TMEM33 antibody BL22301. For blotting immunoprecipitated TMEM33, A305-597A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TMEM33 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMEM33 antibody A305-597A-M (lot A305-597A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TMEM33 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TMEM33 antibody (Product # A305-597A-M (lot A305-597A-T-2) used for IP at 40 µL per reaction. TMEM33 was also immunoprecipitated by a previous lot of this antibody (lot A305-597A-T-1). For blotting immunoprecipitated TMEM33, Product # A305-597A-M was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.