12633-1-AP

antibody from Invitrogen Antibodies

Targeting: DAZL DAZH, DAZL1, DAZLA, MGC26406, SPGYLA

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12633-1-AP

Provider

Invitrogen Antibodies

Product name

DAZL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSTANPETP NSTISREAST QSSSAATSQG YILPEGKIMP NTVFVGGIDV RMDETEIRSF FARYGSVKEV KIITDRTGVS KGYGFVSFFN DVDVQKIVES QINFHGKKLK LGPAIRKQNL CAYHVQPRPL VFNHPPPPQF QNVWTNPNTE TYMQPTTTMN PITQYVQAYP TYPNSPVQVI TGYQLPVYNY QMPPQWPVGE QRSYVVPPAY SAVNYHCNEV DPGAEVVPNE CSVHEATPPS GNGPQKKSVD RSIQTVVSCL FNPENRLRNS VVTQDDYFKD KRVHHFRRSR AMLKSV (1-295 aa encoded by B C027595)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12633-1-AP (DAZL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 12633-1-AP ( DAZL antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DAZL antibody (Product # 12633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DAZL (IP:12633-1-AP, 4ug; Detection:12633-1-AP 1:500) with mouse testis tissue lysate 4400ug.