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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Flow cytometry [1]
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- Product number
- 330401 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#330401, RRID:AB_1089209
- Product name
- Purified anti-human SSEA-4 [MC-813-70]
- Antibody type
- Monoclonal
- Antigen
- Human embryonic carcinoma cell line 2102Ep
- Description
- The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human teratocarcinoma cell line, NCCIT, stained with purified MC-813-70, followed by anti-mouse IgG PE
- Conjugate
- Unconjugated
