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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [8]
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- Product number
- NBP1-85306 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85306, RRID:AB_11014988
- Product name
- Rabbit Polyclonal DAZL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human DAZL antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The human testis-specific proteome defined by transcriptomics and antibody-based profiling.
Djureinovic D, Fagerberg L, Hallström B, Danielsson A, Lindskog C, Uhlén M, Pontén F
Molecular human reproduction 2014 Jun;20(6):476-88
Molecular human reproduction 2014 Jun;20(6):476-88
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAZL Antibody [NBP1-85306] - Analysis in control (vector only transfected HEK293T lysate) and DAZL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Staining of human endometrium shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Analysis in human testis and endometrium tissues. Corresponding DAZL RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Staining of human cerebral cortex shows no positivity in neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Staining of human cerebral cortex, endometrium, prostate and testis using Anti-DAZL antibody NBP1-85306 (A) shows similar protein distribution across tissues to independent antibody NBP1-85307 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Staining of human endometrium shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Staining of human prostate shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DAZL Antibody [NBP1-85306] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
