TA501209

antibody from OriGene

Targeting: EMG1 C2F, Grcc2f, NEP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501209

Provider

OriGene

Proper citation

OriGene Cat#TA501209, RRID:AB_11124761

Product name

Anti-EMG1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Antibody type

Monoclonal

Description

Anti-EMG1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

EMG1

Isotype

IgG

Antibody clone number

OTI1B8

Vial size

100 µl

Concentration

0.97 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EMG1 (RC208885, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EMG1 .

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-EMG1 mouse monoclonal antibody (TA501209) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1 (RC208885 ).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-EMG1 antibody(TA501209),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC