Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 16686-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CKAP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: IFTEVQKRS QKEINDMKAK VASLEESEGN KQDLKALKEA VKEIQTSAKS REWDMEALRS TLQTMESDIY TEVRELVSLK QEQQAFKEAA DTERLALQAL TEKLLRSEES VSRLPEEIRR LEEELRQLKS DSHGPKEDGG FRHSEAFEAL QQKSQGLDSR LQHVEDGVLS MQVASARQTE SLESLLSKSQ EHEQRLAALQ GRLEGLGSSE ADQDGLASTV RSLGETQLVL YGDVEELKRS VGELPSTVES LQKVQEQVHT LLSQDQAQAA RLPPQDFLDR LSSLDNLKAS VSQVEADLKM LRTAVDSLVA YSVKIETNEN NLESAKGLLD DLRNDLDRLF VKVEKIHEKV (254-602 aa encoded by BC082972)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat kidney tissue were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3T3-L1 cells were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3T3-L1 cells were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody 16686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CKAP4 (IP:16686-1-AP, 4ug; Detection:16686-1-AP 1:1000) with HeLa cells lysate 2000ug.