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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP2-60707 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CORO7 Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4degreesC.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CORO7 Antibody [NBP2-60707] - Detection of human CORO7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CORO7 antibody NBP2-60707 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CORO7 Antibody [NBP2-60707] - Detection of human CORO7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CORO7 antibody NBP2-60707 used for IP at 20 ul per reaction. CORO7 was also immunoprecipitated by rabbit anti-CORO7 antibody NBP2-60706. For blotting immunoprecipitated CORO7, NBP2-60707 was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CORO7 Antibody [NBP2-60707] - Detection of human CORO7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CORO7 antibody NBP2-60707 used for IP at 20 ul per reaction. CORO7 was also immunoprecipitated by rabbit anti-CORO7 antibody NBP2-60706. For blotting immunoprecipitated CORO7, NBP2-60707 was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.
