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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA319400 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Pdcd4 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Pdcd4 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PDCD4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.06 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1: 5,000 dilution. Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Affinity purified anti-Pdcd4 was used at a 1:100 dilution to detect Pdcd4 by immunohistochemistry on mouse colon tissue. Tissue was fixed in 4% paraformaldehyde and paraffin embedded. Tissue sections were deparaffinized and treated by trypsinization before staining. Personal Communication. M Young, NCI, Bethesda, MD.
- Validation comment
- IHC
