12587-1-AP

antibody from Invitrogen Antibodies

Targeting: PDCD4 H731

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12587-1-AP

Provider

Invitrogen Antibodies

Product name

PDCD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TPIIQEYFE HGDTNEVAEM LRDLNLGEMK SGVPVLAVSL ALEGKASHRE MTSKLLSDLC GTVMSTTDVE KSFDKLLKDL PELALDTPRA PQLVGQFIAR AVGDGILCNT YIDSYKGTVD CVQARAALDK ATVLLSMSKG GKRKDSVWGS GGGQQSVNHL VKEIDMLLKE YLLSGDISEA EHCLKELEVP HFHHELVYEA IIMVLESTGE STFKMILDLL KSLWKSSTIT VDQMKRGYER IYNEIPDINL DVPHSYSVLE RFVEECFQAG IISKQLRDLC PSRGRKRFVS EGDGGRLKPE SY (169-469 aa encoded by BC026104)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.6 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 MCF-7 cells were stained with 0.2ug PDCD4 antibody (12587-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug.