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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-34395 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TTYH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human kidney tissue lysate.
- Concentration
- 1 mg/mL
Submitted references SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.
TTYH1 and TTYH2 Serve as LRRC8A-Independent Volume-Regulated Anion Channels in Cancer Cells.
Lu Q, Liu J, Zhao S, Gomez Castro MF, Laurent-Rolle M, Dong J, Ran X, Damani-Yokota P, Tang H, Karakousi T, Son J, Kaczmarek ME, Zhang Z, Yeung ST, McCune BT, Chen RE, Tang F, Ren X, Chen X, Hsu JCC, Teplova M, Huang B, Deng H, Long Z, Mudianto T, Jin S, Lin P, Du J, Zang R, Su TT, Herrera A, Zhou M, Yan R, Cui J, Zhu J, Zhou Q, Wang T, Ma J, Koralov SB, Zhang Z, Aifantis I, Segal LN, Diamond MS, Khanna KM, Stapleford KA, Cresswell P, Liu Y, Ding S, Xie Q, Wang J
Immunity 2021 Jun 8;54(6):1304-1319.e9
Immunity 2021 Jun 8;54(6):1304-1319.e9
TTYH1 and TTYH2 Serve as LRRC8A-Independent Volume-Regulated Anion Channels in Cancer Cells.
Bae Y, Kim A, Cho CH, Kim D, Jung HG, Kim SS, Yoo J, Park JY, Hwang EM
Cells 2019 Jun 9;8(6)
Cells 2019 Jun 9;8(6)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human kidney tissue lysate using a TTYH2 polyclonal antibody (Product # PA5-34395) at 1 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TTYH2 in human kidney tissue lysate with TTYH2 Polyclonal Antibody (Product # PA5-34395) at 1 µg/mL
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TTYH2 in human kidney tissue with TTYH2 Polyclonal Antibody (Product # PA5-34395) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TTYH2 in human kidney tissue with TTYH2 Polyclonal Antibody (Product # PA5-34395) at 2.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 VRAC activity is completely abolished in dKO cells, and is restored by expression of TTYH1 or TTYH2. ( a ) Western blot data for TTYH1 and LRRC8A (SWELL1) in SNU-601 and TTYH1 and TTYH2 double-knockout (dKO) cells. ( b ) Representative immunocytochemical images of SNU-601 and dKO cells stained with anti-TTYH2 antibody. ( c ) Representative traces of VRAC currents from dKO cells transfected with GFP controls in isotonic and hypotonic solutions. Notably, VRAC currents were not induced by hypotonic stimulation in these cells. ( d ) Representative traces of VRAC currents from dKO cells transfected with TTYH1-GFP in isotonic and hypotonic solutions. ( e ) Representative traces of VRAC currents from dKO cells transfected with TTYH2-GFP in isotonic and hypotonic solutions. ( f ) Representative traces of VRAC currents from dKO cells co-transfected with TTYH1-GFP and TTYH2-GFP in isotonic and hypotonic solutions. ( g ) Summary bar graph showing current densities in isotonic and hypotonic solutions, as in ( c )-( f ), at +100 mV. Data are presented as means +- SEM (** P < 0.01 and *** P < 0.001).
