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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP6196a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP6196A, RRID:AB_2235395
- Product name
- MMP12 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.56 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum.
Blockade of the c-Jun amino terminal kinase prevents crescent formation and halts established anti-GBM glomerulonephritis in the rat.
LaPan P, Brady J, Grierson C, Fleming M, Miller D, Sypek J, Fu B
BMC pulmonary medicine 2010 Aug 2;10:40
BMC pulmonary medicine 2010 Aug 2;10:40
Blockade of the c-Jun amino terminal kinase prevents crescent formation and halts established anti-GBM glomerulonephritis in the rat.
Ma FY, Flanc RS, Tesch GH, Bennett BL, Friedman GC, Nikolic-Paterson DJ
Laboratory investigation; a journal of technical methods and pathology 2009 Apr;89(4):470-84
Laboratory investigation; a journal of technical methods and pathology 2009 Apr;89(4):470-84
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hMMP12-R406 Pab (Cat. #AP6196a) pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. MMP12(arrow) was detected using the purified Pab;
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hMMP12-R406 Pab (Cat. #AP6196a) pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates. MMP12(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of MMP12 Antibody (C-term) (Cat. #AP6196a) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
