- MA5-24851 - Invitrogen Antibodies MMP12 antibody

Jiang L, Yang M, He S, Li Z, Li H, Niu T, Xie D, Mei Y, He X, Wei L, Huang P, Huang M, Zhang R, Wang L, Li J
BMC cancer 2021 Dec 4;21(1):1297

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MMP12 in Lane 1: A549 whole cell lysate, Lane 2: HeLa whole cell lysate, Lane 3: MCF7 whole cell lysate, Lane 4: THP1 whole cell lysate. Samples were incubated with MMP12 monoclonal antibody (Product # MA5-24851) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MMP12 in Lane 1: A549 whole cell lysate, Lane 2: HeLa whole cell lysate, Lane 3: MCF7 whole cell lysate, Lane 4: THP1 whole cell lysate. Samples were incubated with MMP12 monoclonal antibody (Product # MA5-24851) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MMP12 in Lane 1: A549 whole cell lysate, Lane 2: HeLa whole cell lysate, Lane 3: MCF7 whole cell lysate, Lane 4: THP1 whole cell lysate. Samples were incubated with MMP12 monoclonal antibody (Product # MA5-24851) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of MMP12 in paraffin-embedded mouse lung tissue. Samples were incubated with MMP12 monoclonal antibody (Product # MA5-24851) followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of MMP12 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with MMP12 monoclonal antibody (Product # MA5-24851) followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of MMP12 in A549 cells and unlabeled control (cells without incubation with primary antibody; red). Samples were incubated with MMP12 monoclonal antibody (Product # MA5-24851) at a dilution of 1:50 followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 MMP12 Is Up-regulated in Muscle Tissues and Macrophages of Apc Min/+ Mice. A MMP12 antibody immunostaining in the muscle of healthy individuals. Scale bar: 2 mum. B Immunostaining of MMP12 in muscle tissue of WT mice and Apc Min/+ mice. Scale bar: 5 mum. C Quantification of MM12 expression in gastrocnemius tissue was performed by ImageJ software (40X) (* P < 0.05, data are shown as means +- SD; n = 9 per group). D Representative images of dual immnofluorescent staining of macrophages (F4/80 in green) and MMP12 (in red) in WT mice are shown. The yellow areas in the merged images indicate overlapping localization of the red and green signals, indicated by the white arrows. Scale bars: 20 mum. E Quantification of MMP12 mRNA level in peritoneal macrophages isolated from WT mice and Apc Min/+ mice by qPCR (* P < 0.05; data are shown as the means +- SD; n = 6 per group). F The serum MMP12 levels detected in WT and Apc Min/+ mice at 9, 15, and 24 weeks old by ELISA ( P > 0.05; data are shown as means +- SD; n = 6 per group)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 IL-6 Up-regulates MMP12 in Macrophages. A Schematic diagram of tumor cells (MC38/CT26 cell lines) co-cultured with macrophage cells (RAW264.7 cell lines). All quantifications were performed by image J software for gray scale statistics. B , C Representative western blots showing the secreted MMP12 protein levels from RAW264.7 cell lines (1-2 x 10 5 ) cultured alone or co-cultured with MC38 cell lines (control, 1 x 10 4 , 3 x 10 4 , 5 x 10 4 ). beta-Actin as the internal control. D , E Representative western blots showing the secreted MMP12 protein levels from RAW264.7 cell lines (1-2 x 10 5 ) cultured alone or co-cultured with CT26 cell lines (control, 1 x 10 4 , 3 x 10 4 , 5 x 10 4 ). GAPDH as the internal control. F Schematic diagram of IL-6 treated macrophages. RAW264.7 cells incubated with fresh media were served as untreated negative controls. Western blot was used to detect MMP12 in RAW 264.7 cells and GAPDH as the internal control. G , H RAW264.7 cells (1-2 x 10 5 ) were seeded in 6-well plates and treated with increasing doses of IL-6 (0, 2, 5, 10, 30 ng/mL) for 72 h. I Immune Gene data ( https://www.immgen.org/ImmGenApps.html ) suggest that IL-6 receptor expresses on F480+ macrophages. The colored bars refer to the expression level of IL-6 receptors on macrophages. Red represents high expression of IL-6 receptors, and green represents low expression of IL-6 receptors

MA5-24851