A300-311A

antibody from Invitrogen Antibodies

Targeting: CDK11B CDC2L1, CDK11-p110, CDK11-p46, CDK11-p58

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-311A

Provider

Invitrogen Antibodies

Product name

CDK11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK11 antibody A300-311A (lot A300-311A-3) used for IP at 3 µg per reaction. CDK11 was also immunoprecipitated by a previous lot of this antibody (lot A300-311A-2). For blotting immunoprecipitated CDK11, A300-311A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK11 by western blot. Samples: Whole cell lysate (15 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody A300-311A (lot A300-311A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK11 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDK11 (Cat. No. A300-311A Lot 3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.