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LearnA300-311A
antibody from Invitrogen Antibodies
Targeting: CDK11B
CDC2L1, CDK11-p110, CDK11-p46, CDK11-p58
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- A300-311A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK11 antibody A300-311A (lot A300-311A-3) used for IP at 3 µg per reaction. CDK11 was also immunoprecipitated by a previous lot of this antibody (lot A300-311A-2). For blotting immunoprecipitated CDK11, A300-311A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK11 by western blot. Samples: Whole cell lysate (15 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody A300-311A (lot A300-311A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK11 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDK11 (Cat. No. A300-311A Lot 3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
