Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF3315 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DSCAM-L1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human DSCAM-L1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human DSCAM is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8TD84
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references DSCAM promotes axon fasciculation and growth in the developing optic pathway.
Bruce FM, Brown S, Smith JN, Fuerst PG, Erskine L
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 14;114(7):1702-1707
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 14;114(7):1702-1707
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of DSCAM-L1 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human DSCAM-L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3315, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).