PA5-69312

antibody from Invitrogen Antibodies

Targeting: TMEM220

Western blot

Antibody data

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Validation data

Product number: PA5-69312 - Provider product page
Provider: Invitrogen Antibodies
Product name: TMEM220 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This target displays homology in the following species: Cow: 91%; Dog: 100%; Guinea Pig: 86%; Horse: 92%; Human: 100%; Mouse: 82%; Rabbit: 100%; Rat: 83%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

TMEM220 protein structure - PA5-69312 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 TMEM220 is a target of TMEM220-AS1. (A) HCC cells were transfected with pcDNA3.1 vector carrying TMEM220-AS1 or with empty vector (EV) and TMEM220 mRNA was detected using RT-qPCR. (B) Western blotting analysis revealed that TMEM-220-AS1 overexpression upregulated TMEM220 protein levels in HCC cells. (C) The levels of TMEM220 mRNA were measured in 80 pairs of HCC and nontumor (NT) tissues. (D) TMEM220-AS1 expression was assessed in MIHA, HCCLM3, Huh7, HepG2, Hep3B, and MHCC97H cells. (E) TMEM220-AS1 was positively associated with TMEM220 mRNA expression in HCC tissue samples. (F) The survival of HCC patients with low versus high TMEM220 expression was compared. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 TMEM220 suppresses the migration, invasion, and proliferation of HCC cells. (A) MHCC97H and HCCLM3 cells were transfected with pcDNA3.1 vector carrying TMEM220 or with empty vector (EV) and TMEM220 analyzed using western blotting analysis. (B) TMEM220 overexpression suppressed HCC cell viability, as determined using CCK-8 assay. (C) EdU assay confirmed that TMEM220 overexpression weakened HCC cell proliferation. (D) The number of migrated and invaded HCC cells was decreased by TMEM220 overexpression. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 TMEM220 knockdown reverses the inhibitory effect of TMEM220-AS1 in HCCLM3 cells. (A) Western blot analysis showed that TMEM220 levels were reduced by TMEM220 shRNA (shTMEM220) in TMEM220-AS1-overexpressing HCCLM3 cells. ( B ) MTT, ( C ) EdU, and ( D ) transwell analyses demonstrated that TMEM220 knockdown reversed the suppressive role of TMEM220-AS1 in HCCLM3 cells. * P