PA1-846

antibody from Invitrogen Antibodies

Targeting: NCOA2 bHLHe75, GRIP1, KAT13C, NCoA-2, TIF2

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-846

Provider

Invitrogen Antibodies

Product name

SRC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-846 detects glucocorticoid receptor interacting protein-1 (GRIP1) from hamster and human cells.

Concentration

1 mg/mL

References

Submitted references

Regulation of GRIP1 and CBP Coactivator activity by Rho GDI modulates estrogen receptor transcriptional enhancement.

Su LF, Wang Z, Garabedian MJ
The Journal of biological chemistry 2002 Oct 4;277(40):37037-44

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Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones.

Rogatsky I, Zarember KA, Yamamoto KR
The EMBO journal 2001 Nov 1;20(21):6071-83

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Multiple receptor interaction domains of GRIP1 function in synergy.

Schmidt S, Baniahmad A, Eggert M, Schneider S, Renkawitz R
Nucleic acids research 1998 Mar 1;26(5):1191-7

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Multiple receptor interaction domains of GRIP1 function in synergy.

Schmidt S, Baniahmad A, Eggert M, Schneider S, Renkawitz R
Nucleic acids research 1998 Mar 1;26(5):1191-7

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GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.

Hong H, Kohli K, Garabedian MJ, Stallcup MR
Molecular and cellular biology 1997 May;17(5):2735-44

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GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.

Hong H, Kohli K, Garabedian MJ, Stallcup MR
Molecular and cellular biology 1997 May;17(5):2735-44

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunolocalization of GRIP1 in CHO cells using Product # PA1-846.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GRIP1 was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GRIP1 Rabbit Polyclonal Antibody (Product # PA1-846) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunolocalization of GRIP1 in CHO cells using Product # PA1-846.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL