PA5-98577

antibody from Invitrogen Antibodies

Targeting: RTN4RL2 NgR2, NGRH1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-98577

Provider

Invitrogen Antibodies

Product name

RTN4RL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RTN4RL2 using a RTN4RL2 Polyclonal antibody (Product # PA5-98577). Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate. All lanes: RTN4RL2 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 47 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RTN4RL2 in MCF-7 cells using a RTN4RL2 polyclonal antibody (Product # PA5-98577) at a dilution of 1:50. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RTN4RL2 in paraffin embedded human liver tissue using a RTN4RL2 polyclonal antibody (Product # PA5-98577) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.