NBP2-12812

antibody from Novus Biologicals

Targeting: GIGYF2 GYF2, KIAA0642, PARK11, PERQ2, PERQ3, TNRC15

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-12812

Provider

Novus Biologicals

Product name

Rabbit Polyclonal PERQ2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes.

Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild K, Béthune J
Nature communications 2017 Jun 6;8:15690

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: PERQ2 Antibody [NBP2-12812] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PERQ2 antibody NBP2-12811 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PERQ2 was also immunoprecipitated by rabbit anti-PERQ2 antibodies NBP2-12812 and NBP2-12813, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).