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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM31050PU-N - Provider product page
- Provider
- OriGene
- Product name
- ENO3 mouse monoclonal antibody, clone 5D1, Purified
- Antibody type
- Monoclonal
- Description
- ENO3 mouse monoclonal antibody, clone 5D1, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ENO3
- Isotype
- IgG
- Antibody clone number
- 5D1
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ENO3 monoclonal antibody (M01), clone 5D1 Western Blot analysis of ENO3 expression in HeLa.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M01), clone 5D1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody (M01), clone 5D1 .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ENO3 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ENO3 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC
