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LearnH00002027-M01
antibody from Novus Biologicals
Targeting: ENO3
Western blot ELISA Immunocytochemistry
Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- H00002027-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002027-M01, RRID:AB_2099465
- Product name
- Mouse Monoclonal ENO3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ENO3 - enolase 3 (beta, muscle)
- Reactivity
- Human, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Beef tenderness and intramuscular fat proteomic biomarkers: Effect of gender and rearing practices.
Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect.
Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed.
Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses.
Unraveling proteome changes of Holstein beef M. semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry.
Coherent correlation networks among protein biomarkers of beef tenderness: What they reveal.
Human skeletal muscle fibre contractile properties and proteomic profile: adaptations to 3 weeks of unilateral lower limb suspension and active recovery.
Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle.
The time course of the adaptations of human muscle proteome to bed rest and the underlying mechanisms.
Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C.
Functional analysis of beef tenderness.
Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle.
Identification of alpha-enolase as an autoantigen in lung cancer: its overexpression is associated with clinical outcomes.
Picard B, Gagaoua M, Al Jammas M, Bonnet M
Journal of proteomics 2019 May 30;200:1-10
Journal of proteomics 2019 May 30;200:1-10
Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect.
Picard B, Gagaoua M, Al-Jammas M, De Koning L, Valais A, Bonnet M
PeerJ 2018;6:e4891
PeerJ 2018;6:e4891
Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed.
Gagaoua M, Bonnet M, De Koning L, Picard B
Meat science 2018 Nov;145:308-319
Meat science 2018 Nov;145:308-319
Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses.
Gagaoua M, Bonnet M, Ellies-Oury MP, De Koning L, Picard B
Food chemistry 2018 Jun 1;250:245-252
Food chemistry 2018 Jun 1;250:245-252
Unraveling proteome changes of Holstein beef M. semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry.
Yu Q, Wu W, Tian X, Hou M, Dai R, Li X
Journal of proteomics 2017 Feb 10;154:85-93
Journal of proteomics 2017 Feb 10;154:85-93
Coherent correlation networks among protein biomarkers of beef tenderness: What they reveal.
Gagaoua M, Terlouw EM, Boudjellal A, Picard B
Journal of proteomics 2015 Oct 14;128:365-74
Journal of proteomics 2015 Oct 14;128:365-74
Human skeletal muscle fibre contractile properties and proteomic profile: adaptations to 3 weeks of unilateral lower limb suspension and active recovery.
Brocca L, Longa E, Cannavino J, Seynnes O, de Vito G, McPhee J, Narici M, Pellegrino MA, Bottinelli R
The Journal of physiology 2015 Dec 15;593(24):5361-85
The Journal of physiology 2015 Dec 15;593(24):5361-85
Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle.
Picard B, Gagaoua M, Micol D, Cassar-Malek I, Hocquette JF, Terlouw CE
Journal of agricultural and food chemistry 2014 Oct 8;62(40):9808-18
Journal of agricultural and food chemistry 2014 Oct 8;62(40):9808-18
The time course of the adaptations of human muscle proteome to bed rest and the underlying mechanisms.
Brocca L, Cannavino J, Coletto L, Biolo G, Sandri M, Bottinelli R, Pellegrino MA
The Journal of physiology 2012 Oct 15;590(20):5211-30
The Journal of physiology 2012 Oct 15;590(20):5211-30
Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C.
Uys GM, Ramburan A, Loos B, Kinnear CJ, Korkie LJ, Mouton J, Riedemann J, Moolman-Smook JC
BMC cell biology 2011 May 10;12:18
BMC cell biology 2011 May 10;12:18
Functional analysis of beef tenderness.
Guillemin N, Bonnet M, Jurie C, Picard B
Journal of proteomics 2011 Dec 21;75(2):352-65
Journal of proteomics 2011 Dec 21;75(2):352-65
Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle.
Chaze T, Meunier B, Chambon C, Jurie C, Picard B
Animal : an international journal of animal bioscience 2009 Jul;3(7):980-1000
Animal : an international journal of animal bioscience 2009 Jul;3(7):980-1000
Identification of alpha-enolase as an autoantigen in lung cancer: its overexpression is associated with clinical outcomes.
Chang GC, Liu KJ, Hsieh CL, Hu TS, Charoenfuprasert S, Liu HK, Luh KT, Hsu LH, Wu CW, Ting CC, Chen CY, Chen KC, Yang TY, Chou TY, Wang WH, Whang-Peng J, Shih NY
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Oct 1;12(19):5746-54
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Oct 1;12(19):5746-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ENO3 Antibody (5D1) [H00002027-M01] - Analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi ( Cat # H00002027-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody (M01), clone 5D1 (Cat # H00002027-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ENO3 Antibody (5D1) [H00002027-M01] - Analysis of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M01), clone 5D1.Lane 1: ENO3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ENO3 Antibody (5D1) [H00002027-M01] - ENO3 monoclonal antibody (M01), clone 5D1 Analysis of ENO3 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ENO3 Antibody (5D1) [H00002027-M01] - Detection limit for recombinant GST tagged ENO3 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ENO3 Antibody (5D1) [H00002027-M01] - Analysis of monoclonal antibody to ENO3 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ENO3 Antibody (5D1) [H00002027-M01] - Analysis of monoclonal antibody to ENO3 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ENO3 Antibody (5D1) [H00002027-M01] - Analysis of monoclonal antibody to ENO3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
