H00002027-M04

antibody from Abnova Corporation

Targeting: ENO3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002027-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002027-M04, RRID:AB_1136928

Product name

ENO3 monoclonal antibody (M04), clone 3C6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant ENO3.

Antigen sequence

MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAV
PSGASTGIYEALELRDGDKGRYLGKGVLKAVENIN
STLGPALLQKKLSVADQEKVDKFMIELDGTENKSK
FGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGN
PDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGA
SSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDE
GGFAPNILENNEALELLKTAIQAAGYPDKVVIGMD
VAASEFYRNGKYDLDFKSPDDPARHITGEKLGELY
KSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQI
VGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSV
TESIQACKLAQSNGWGVMVSHRSGETEDTFIADLV
VGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK
AIFAGRKFRNPKAK


Isotype

IgG

Antibody clone number

3C6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M04), clone 3C6.Lane 1: ENO3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ENO3 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol