V9337

antibody from NSJ Bioreagents

Targeting: CLU APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V9337

Provider

NSJ Bioreagents

Product name

Clusterin Antibody / Apolipoprotein J / APO-J

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

CLU/4727

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the CLU antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of human testis tissue lysate using CLU antibody (clone CLU/4727). Predicted molecular weight: 75-80 kDa (heterodimer precursor), 36-39 kDa (alpha subunit), 34-36 kDa (beta subunit).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human adrenal gland with CLU antibody (clone CLU/4727). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CLU antibody (clone CLU/4727). These results demonstrate the foremost specificity of the CLU/4727 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free CLU antibody (clone CLU/4727) as confirmation of integrity and purity.