Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-18910 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18910, RRID:AB_1625759
- Product name
- Rabbit Polyclonal KHSRP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The KH-type splicing regulatory protein (KSRP) regulates type III interferon expression post-transcriptionally.
Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism.
KSRP modulation of GAP-43 mRNA stability restricts axonal outgrowth in embryonic hippocampal neurons.
miR-27b targets KSRP to coordinate TLR4-mediated epithelial defense against Cryptosporidium parvum infection.
Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cells.
Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma.
Schmidtke L, Schrick K, Saurin S, Käfer R, Gather F, Weinmann-Menke J, Kleinert H, Pautz A
The Biochemical journal 2019 Jan 31;476(2):333-352
The Biochemical journal 2019 Jan 31;476(2):333-352
Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism.
Agca C, Boldt K, Gubler A, Meneau I, Corpet A, Samardzija M, Stucki M, Ueffing M, Grimm C
BMC biology 2015 Apr 25;13:30
BMC biology 2015 Apr 25;13:30
KSRP modulation of GAP-43 mRNA stability restricts axonal outgrowth in embryonic hippocampal neurons.
Bird CW, Gardiner AS, Bolognani F, Tanner DC, Chen CY, Lin WJ, Yoo S, Twiss JL, Perrone-Bizzozero N
PloS one 2013;8(11):e79255
PloS one 2013;8(11):e79255
miR-27b targets KSRP to coordinate TLR4-mediated epithelial defense against Cryptosporidium parvum infection.
Zhou R, Gong AY, Eischeid AN, Chen XM
PLoS pathogens 2012;8(5):e1002702
PLoS pathogens 2012;8(5):e1002702
Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cells.
Neff AT, Lee JY, Wilusz J, Tian B, Wilusz CJ
Genome research 2012 Aug;22(8):1457-67
Genome research 2012 Aug;22(8):1457-67
Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma.
Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, Takekawa M, Yoshida H, Omata M, Koike K
PloS one 2011;6(9):e24359
PloS one 2011;6(9):e24359
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: KHSRP Antibody [NBP1-18910] - Simple Western lane view shows a specific band for KHSRP in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. KSRP was also immunoprecipitated by rabbit anti-KSRP antibody NBP1-18911.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiKSRP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: KHSRP Antibody [NBP1-18910] - KSRP immunostaining in mouse cerebral cortex and hippocampus. Cell were stained with Novus KSRP. This KSRP antibody was also used for immunoprecipation and western blot analyses. Image from confirmed customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-KSRP used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-KSRP used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: KHSRP Antibody [NBP1-18910] - Immunofluorescence of ILF3 and KHSRP in the retina. Retinal sections were prepared from control mice and from mice at one day after light exposure and stained for GS (green) and KHSRP (red). Image collected and cropped by CiteAb from the following publication (http://bmcbiol.biomedcentral.com/articles/10.1186/s12915-015-0137-1), licensed under a CC-BY licence.
