AF-466-NA
antibody from R&D Systems
Targeting: CXCL10
C7, crg-2, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB10
Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
Reference
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- Product number
- AF-466-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL10/IP-10/CRG-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human CXCL10 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P17515
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts.
Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease.
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Brain inflammation is accompanied by peripheral inflammation in Cstb -/- mice, a model for progressive myoclonus epilepsy.
CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.
Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
Divergent effects of T cell costimulation and inflammatory cytokine production on autoimmune peripheral neuropathy provoked by Aire deficiency.
Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture.
CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice.
CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice.
Viral infection of the lungs through the eye.
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Muramatsu M, Gao L, Peresie J, Balderman B, Akakura S, Gelman IH
Oncotarget 2017 Sep 19;8(41):70281-70298
Oncotarget 2017 Sep 19;8(41):70281-70298
Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease.
Li X, Yao QY, Liu HC, Jin QW, Xu BL, Zhang SC, Tu CT
Journal of cellular and molecular medicine 2017 Oct;21(10):2370-2385
Journal of cellular and molecular medicine 2017 Oct;21(10):2370-2385
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Thaney VE, O'Neill AM, Hoefer MM, Maung R, Sanchez AB, Kaul M
Scientific reports 2017 Apr 20;7:46514
Scientific reports 2017 Apr 20;7:46514
Brain inflammation is accompanied by peripheral inflammation in Cstb -/- mice, a model for progressive myoclonus epilepsy.
Okuneva O, Li Z, Körber I, Tegelberg S, Joensuu T, Tian L, Lehesjoki AE
Journal of neuroinflammation 2016 Nov 28;13(1):298
Journal of neuroinflammation 2016 Nov 28;13(1):298
CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.
Dai Z, Xing L, Cerise J, Wang EH, Jabbari A, de Jong A, Petukhova L, Christiano AM, Clynes R
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1089-99
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1089-99
Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
Mills Ko E, Ma JH, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika AM, Pleasure D
Journal of neuroinflammation 2014 Jun 12;11:105
Journal of neuroinflammation 2014 Jun 12;11:105
Divergent effects of T cell costimulation and inflammatory cytokine production on autoimmune peripheral neuropathy provoked by Aire deficiency.
Zeng XL, Nagavalli A, Smith CJ, Howard JF, Su MA
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 15;190(8):3895-904
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 15;190(8):3895-904
Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
Leconet W, Petit P, Peraldi-Roux S, Bresson D
Molecular therapy : the journal of the American Society of Gene Therapy 2012 Dec;20(12):2315-25
Molecular therapy : the journal of the American Society of Gene Therapy 2012 Dec;20(12):2315-25
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture.
King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP 3rd, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE
Circulation 2009 Jan 27;119(3):426-35
Circulation 2009 Jan 27;119(3):426-35
CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice.
Maeno T, Houghton AM, Quintero PA, Grumelli S, Owen CA, Shapiro SD
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8090-6
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8090-6
CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice.
Maeno T, Houghton AM, Quintero PA, Grumelli S, Owen CA, Shapiro SD
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8090-6
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):8090-6
Viral infection of the lungs through the eye.
Bitko V, Musiyenko A, Barik S
Journal of virology 2007 Jan;81(2):783-90
Journal of virology 2007 Jan;81(2):783-90
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Fujita H, Asahina A, Komine M, Tamaki K
Cellular immunology 2007 Feb;245(2):70-9
Cellular immunology 2007 Feb;245(2):70-9
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CXCL10/IP-10/CRG-2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL10/IP-10/CRG-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL10/IP-10/CRG-2 in Mouse Thymus. CXCL10/IP-10/CRG-2 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL10/CRG-2 and Neutral-ization by Mouse CXCL10/ CRG-2 Antibody. Recombinant Mouse CXCL10/ CRG-2 (Catalog # 466-CR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/ CRG-2 (0.5 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Mouse CXCL10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA). The ND50 is typically 5-25 µg/mL.