PA5-46999

antibody from Invitrogen Antibodies

Targeting: CXCL10 C7, crg-2, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB10

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46999

Provider

Invitrogen Antibodies

Product name

CXCL10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse CRG-2, recombinant human (rh) MIG, and rhGRO gamma. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA of CXCL10 in recombinant Human CXCL10/IP‚10/CRG‚2 protein. Samples were incubated in CXCL10 polyclonal antibody (Product # PA5-46999). Protein was serially diluted 2-fold. Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP followed by Substrate Solution and stopping the enzymatic reaction with Stop Solution.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CXCL10/IP-10 was detected in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46999) for 3 hours at room temperature. Cells were stained with the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of CXCL10 in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs). Samples were incubated in CXCL10 polyclonal antibody (Product # PA5-46999) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of CXCL10 in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs). Samples were incubated in CXCL10 polyclonal antibody (Product # PA5-46999) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CXCL10 in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated with CXCL10 polyclonal antibody (Product # PA5-46999) using a dilution of 1 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CXCL10 in BaF3 mouse pro‚B cell line transfected with human CXCR3. Samples were incubated in CXCL10 polyclonal antibody (Product # PA5-46999). Recombinant Human CXCL10/IP‚10 chemoattracts the BaF3 mouse pro‚B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL10/IP‚10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL10/IP‚10 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1-4 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CXCL10 is shown by the decrease in mean RFU (relative fluorescent units - measured by Resazurin - measure of chemotaxis) with increasing concentrations of CXCL10 Polyclonal Antibody (PA5-46999).